Acid Mucopolysaccharides in Normal Serum1

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Acid mucopolysaccharides in normal serum.

Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1, 2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the p...

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Quantitative Colorimetric Assay of Acid Mucopolysaccharides

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

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Comparison of Cell Coat Acid Mucopolysaccharides of Normal Liver and Various Ascites Hepatoma Cells1

avoiding cytolysis or membrane disruption. After incuba tion, the mixtures were centrifuged, and 105,000 x g supernatants were subjected to gel filtration. The macromolecular peak fractions thus obtained contained about 2% radio activity of the whole cells or 50% radioactivity of the plasma membranes. They showed several physical and chemical features characteristic of acid mucopolysaccharides....

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Quantitative Colorimetric Assay of Acid Mucopolysaccharides

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

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Quantitative colorimetric assay of acid mucopolysaccharides.

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 1957

ISSN: 0021-9738

DOI: 10.1172/jci103531